Abstract:[Objective] To evaluate the effect of matrix-induced autologous chondrocytes implantation under arthroscopy in the repair of knee cartilage defects. [Methods] A total of 12 patients (12 knees, 8 males and 4 females, the average age was 30, the average BMI index was 25.3±4.25kg/m 2), were treated with non-suture matrix-induced autologous implantation（MACI） under all arthroscopy in the first affiliated Hospital of Shenzhen University from September 2016 to February 2018. The lysholm score, VAS score and Z value depending on T2-MAP MRI were calculated preoperation, postoperative (PO) 3 ,6 , 9 and 12 months after operation. [Results] All patients were followed up effectively. The graft adheres well 3 months after operation, edema signal disappeared at 6 months after operation, and the signal of cartilage graft remained stable in the following 1 year. One year after operation, the value of T2-MAP Z value was significantly higher than that before operation(p＜0.05), and at 6 months after operation, it was significantly higher than that at 3 months after operation (0 53 ±0 23 vs 0 85 ±0 94, p < 0 05).The lysholm score increased significantly 3 months after operation (62.08 ±6.51 vs 82.75±3.49, p<0.05).The lysholm scores at 12 months after operation was significantly higher than that at 3 months (90.67 ±2.06 vs 62.08 ±6.51, p < 0.05).There was no significant difference between 12 months and 9 months after operation (p > 0.05).The VAS scores of the patients decreased significantly 1 year after operation (0.25 ±0.45 vs 5.17 ±1.03, p < 0.05)..[Conclusion] Matrix-induced autogenous chondrocyte transplantation under total arthroscopy could effectively accelerate the recovery of knee joint, improve the symptoms of discomfort before operation, without serious complications, and can repair the cartilage injury very well.