文章摘要
牛宁奎,纳莉,丁惠强,吕爱君,何进文,吴龙云,杨宗强,师志云,施建党.长链非编码 RNA 和 mRNA 在脊柱转移癌表达的差异[J].,2019,27(17):1593-1599
长链非编码 RNA 和 mRNA 在脊柱转移癌表达的差异
Differential expression of long non-coding RNA and mRNA in spinal metastatic carcinoma
投稿时间:2018-12-23  
DOI:
中文关键词: 脊柱转移癌, 长链非编码 RNA, 表达谱, 微阵列分析
英文关键词: spinal metastases, long non-coding RNA, expression profile, microarray chip
基金项目:
作者单位
牛宁奎  
纳莉  
丁惠强  
吕爱君  
何进文  
吴龙云  
杨宗强  
师志云  
施建党  
摘要点击次数: 19
全文下载次数: 0
中文摘要:
      摘要:[目的] 利用基因芯片筛选脊柱转移癌患者组织中差异表达的 lncRNAs 和 mRNAs,分析 lncRNAs 在脊柱 转移癌中可能的分子机制。[方法]选取 2016 年宁夏医科大学总医院就诊经病理证实的 3 例脊柱转移癌患者和 3 例 正常对照患者。利用 LncPathTM Human Cancer Array (8×15 K, Arraystar) 芯片检测患者和正常对照组织的 lncRNAs 和 mRNAs、GO 及 KEGG pathway,分析差异表达的 lncRNAs 和 mRNAs 的功能分布。[结果]脊柱转移癌患者中差异表达 的 lncRNAs 有 326 条,差异表达的 mRNAs 有 775 条。GO 分析发现差异表达的 mRNAs 主要参与细胞增殖调节、生物 细胞过程负调控、局灶性粘连、细胞-基质粘连等。Pathway 分析发现差异表达的 mRNAs 主要参与癌症途径、AGERAGE 信号通路与糖尿病并发症、细胞因子及其受体相互作用、慢性粒细胞白血病等。[结论]脊柱转移癌患者椎体组 织中差异表达的 lncRNAs 可能与脊柱转移癌的发生和发展密切相关,未来可能成为脊柱转移癌潜在治疗靶点。
英文摘要:
      Abstract: [Objective] To screen the differential expressions of lncRNAs and mRNA in the tissues of spinal metastases by gene chip, and to analyze the possible molecular mechanisms of lncRNAs in spinal metastases. [Methods] Three patients with spinal metastases confirmed by pathology in Ningxia Medical University General Hospital in 2016 and three normal controls were selected. LncPathTM Human Cancer Array (8×15 K, Arraystar) chip was used to detect lncRNAs and mRNAs in the meta? static tumor and normal tissues. In addition, GO and KEGG pathway analysis were conducted to show the differences in expres? sions of lncRNAs and mRNAs. [Results] The spinal metastatic tumor had 326 IncRNA and 775 mRNA in differential expres? sions compared with the normal tissues. GO analysis found that differentially expressed mRNAs were mainly involved in cell proliferation regulation, negative regulation of biological cell processes, focal adhesions, and cell- matrix adhesions. Pathway analysis found that differentially expressed mRNAs were mainly involved in the cancer pathway, AGE-RAGE signaling path? way and diabetes complications, cytokine and its receptor interaction, chronic myeloid leukemia. [Conclusion] The differential expression of lncRNAs in vertebral tissue of patients with spinal metastases may be closely related to the occurrence and devel? opment of spinal metastases, and may become a potential therapeutic target for spinal metastases in the future.
查看全文   查看/发表评论  下载PDF阅读器
关闭