Objective: To evaluate the application of Bone Scanning Imaging in the diagnosis of multiple osteoporotic vertebral compression fractures (MOVCFs) in patients with contraindications to magnetic resonance imaging (MRI). Methods: A retrospective study was conducted in 14 patients with MOVCFs with contraindications to MRI in our hospital from January 2013 to June 2022. There were 8 females and 6 males, ranging in age from 57 to 79 years, with a mean age of 67.9 years, followed up 12 to 36 months, with a mean follow-up of 22.6 months. All patients undergo x-rays, CT, and bone scan imaging to identify the painful vertebra. Verbroplasty is performed in the painful vertebral body. Clinical outcomes were assessed using visual analogue scores (VAS) and Oswestry indices (ODIs) during preoperative, postoperative, and final follow-up assessments. Results: Bone Scanning Imaging showed that 13 of the 14 patients (32 vertebral bodies in total) showed concentrated ingestion, 17 vertebral bodies were identified as painful vertebral, and all patients successfully completed the operation without surgical complications. Bone Scanning Imaging showed a positivity rate of 53.1%. Compared with the preoperative symptoms, VAS and ODI were significantly improved in the first and last postoperative follow-up patients, but there was no significant difference between VAS and ODI on the first and last postoperative follow-up. Conclusion: For patients with MOVCFs with contraindications to MRI, Bone Scanning Imaging is an effective method to determine painful vertebral bodies. Keywords: Bone Scanning Imaging, osteoporosis multiple vertebral compression fracture, vertebroplasty