Microwave hyperthermia is widely used in a variety of clinical and sports rehabilitation environments to treat skeletal muscle injuries. Microwave hyperthermia can not only reduce cell damage and protein degradation after soft tissue injury, but also increase the expression of heat shock protein (HSP) and up-regulate the expression of genes related to muscle growth and differentiation. Microwave heating after muscle injury usually reduces muscle atrophy and oxidative stress in local tissue, and promotes the growth or regeneration of skeletal muscle. In clinical experiments, microwave hyperthermia before exercise can relieve muscle soreness, and human muscle after hyperthermia can enhance strength training and improve muscle quality.