[Objective] To investigate the clinical efficacy of percutaneous fixation combined with channel-assisted implantation of lesion removal loaded calcium sulfate particle implantation for lumbar brucellosis. [Methods] A total of 19 patients, 12 males and 7 females, aged 30~66 years, mean （47.31 ± 11.13） years ，were collected from From July 2017 to July 2021. After regular anti-brucellosis treatment for 6 weeks, the disease was evaluated preoperative. After the evaluation, posterior percutaneous pedicle screw fixation combined with channel-assisted lesion removal and drug-loaded calcium sulfate particle placement were performed. After the operation, regular antibacterial treatment was continued for 8~12weeks. Besides, they were evaluated regularly and followed-up for 18 months. To compare the ESR, CRP, VAS, spinal injury ASIA grade and Bridwell osteointegration before surgery, 1 week after surgery and at the last follow-up.[Results] Operation time (167.57±20.71) min; Blood loss (193.15±57.51) ml; The ESR, CRP, and VAS scores were significantly lower at the last follow-up compared with the preoperative,the difference was statistically significant (F=543.73, respectively, P<0.01; F=57.67, P<0.01; F=135.37, P<0.01). Bone union was achieved in all patients at the last follow-up, one ASIA grade was increased from B to D, two from C to E, eight from D to E, and one case remained in grade D after surgery. There were no vascular and nerve injury complications during the operation, and there were no cases of infection recurrence or failure of internal fixation. [Conclusion] On the basis of standardized antimicrobial therapy, percutaneous fixation combined with drug-loaded calcium sulfate particle implantation can achieve immediate stability, and continuously release antibiotics locally, which is conducive to infection control and has definite clinical effect.