[Objective] To explore the factors related to vertebral fracture (VF) in Duchenne muscular dystrophy (DMD) in children. [Methods] A retrospective study was conducted on the clinical data of DMD patients who admitted to the multidisciplinary joint outpatient de- partment of DMD in General Hospital of PLA from July 2021 to September 2022. The children were divided into VF group and non-VF group according to the presence or absence of VF. The factors related to VF were analyzed by univariate comparison, binary multiple logic analysis and receiver operating characteristic (ROC) . [Results] A total of 93 children were included in this study, and all of them were male, aged from 3.9 to 15.4 years old with an average age of (8.7±2.6) years old. Among them, 19 cases proved VF, accounting for 20.4%; while the remaining 74 cases were of non-VF, accounting for 79.6%. Among them, 56 children were treated with hormone therapy, 17 cases (30.4%) were with VF and 39 cases (69.6%) were with non-VF. In term of univariate comparison, the VF group had significantly greater the time of hormone taking, age and supine-standing position time, whereas significantly less lumbar bone mineral density Z value (LBMD-Z) than the non-VF group, with statistical significance (P＜0.05) . As results of logistic regression, hormone taking duration (OR=2.880, P=0.001) and supine-standing time (OR=1.115, P=0.015) were independent risk factors for VF, while LBMD-Z value (OR=0.102, P＜0.001) , age (OR=0.511, P=0.021) was the protective factor for VF. In term of receiver operating characteristic (ROC) analysis, the aera under curve (AUC) of LBMD-Z value and hor- mone administration duration ranged from 0.7 to 0.9, which had a good value in predicting VF. [Conclusion] The VF in DMD patients is the result of the combined action of multiple factors. Targeted measures to the impacting factors might be helpful to reduce the occurrence of VF.