Abstract:Abstract: Objective To investigate the effects of the methods of effects of tonifying kidney and spleen, benefiting essence and filling marrow on bone metabolism and bone biomechanics in osteoporosis model rats. Methods Wistar rats of clean grade were selected, and 60 rats were divided into operation group (48 rats) and normal group (12 rats) according to the random grouping method. The rat's bilateral ovaries were removed to prepare an osteoporosis model. 7 days after the operation, the surviving rats in the operation group were randomly divided into 4 groups, each with 12 rats: Sham operation group, model group, Yougui Pill group and experimental group. After 12 weeks of intervention, the rats were killed after collecting urine within 24 hours. Aortic blood and left femur were taken for metabolic indexes and bone biomechanics tests. Results (1) There was no significant difference in the concentrations of serum Ca and P in each group (P > 0.05). The level of (alkaline phosphatase,ALP)ALP in the model group was significantly higher than that in the normal group and the sham operation group (P < 0.001). There was no significant difference in ALP level between Yougui Pill group and normal group and sham operation group (P > 0.05). The level of ALP in the experimental group was significantly lower than that in other groups (P < 0.001). (2) Urinary Ca / Cr and urinary P / Cr in the model group were significantly higher than those in the normal group and sham operation group (P < 0.001). There was no significant difference in Ca / Cr and P / Cr between Yougui Pill group and normal group and sham operation group (P > 0.05). Ca / Cr and P / Cr in the experimental group were significantly lower than those in other groups (P < 0.05). (3) PINP and BMP-2 in the model group were lower than those in other groups, and the difference was statistically significant (P < 0.001). The concentrations of PINP and BMP-2 in the experimental group were higher than those in Yougui Pill group and model group (P < 0.05), but there was no significant difference with normal group and sham operation group (P > 0.05). (4) The bone mineral density of the model group was significantly lower than that of other groups (P < 0.001). The bone mineral density of the experimental group was significantly higher than that of the model group and Yougui Pill group (P > 0.05), but there was no significant difference with the normal group and sham operation group (P > 0.05). The bone strength of the model group was significantly lower than that of other groups (P < 0.001).. Conclusion The method of tonifying kidney and spleen, benefiting essence and filling marrow has the effect of anti-osteoporosis, and the mechanism may be related to adjusting the intestinal micro ecological environment, reducing the loss of bone minerals, up regulating the expression of BMP-2 in bone tissue and increasing the activity of osteoblasts.