Ribosome biogenesis (RiBi) plays an important role in the growth and proliferation of cell. Currently, RiBi is considered a close relationship with malignant tumor, on other hand, interfering the process of RiBi will inhibit tumor cells, which in some respects have been convinced that could be potential therapy targets. In addition, some RNA Pol I inhibitors have been developed and tested clinically. The research of RiBi may provide new therapeutic approaches for malignant therapy.