The second affiliated Hospital of Inner Mongolia Medical University
目的:以磷酸钙骨水泥/纤维蛋白胶作为复合材料载体，制备携带骨髓间充质干细胞及力生长因子的组织工程化骨，并观察利用此植入材料体内修复大鼠骨质疏松性骨缺损的效果。 方法:制作大鼠骨质疏松性骨缺损模型，将体外构建的复合骨支架材料（C组：CPC/FG/MGF/BMSCs）植入骨质疏松性骨缺损区，与组A（植入CPC/FG材料）及组B（植入CPC/FG/BMSCs）进行对照实验。术后4周，8周取材，通过放射学、组织学染色、免疫组化等方法观察骨缺损修复情况。 结果: C组大鼠的骨修复进程在术后4周及8周时，相较于A、B两组发展更快；而免疫组化实验结果分析中显示：术后4周时C组相较于B组及A组有着更高的ALP阳性表达（P＜0.05）；术后8周时C组及B组的ALP阳性表达均显著高于A组（P＜0.05），同时，C组与B组间并不存在显著性差异（P＞0.05）。 结论:经骨质疏松大鼠桡骨缺损模型体内修复实验进行的初步评测，表明：负载力生长因子与骨髓间充质干细胞的磷酸钙骨水泥/纤维蛋白胶复合支架材料可能具有较好的修复骨质疏松性骨缺损的能力，值得进一步研究其机理及效能。
Objective: To prepare tissue engineered bone with bone marrow mesenchymal stem cells and force growth factor using calcium phosphate cement / fibrin glue as composite carrier, and to observe the effect of this implant on repairing osteoporotic bone defect in rats. Methods: The rat model of osteoporotic bone defect was made, and the composite bone scaffold constructed in vitro (group C: CPC/FG/MGF/BMSCs) was implanted into the area of osteoporotic bone defect, and compared with group A (implanted CPC/FG material) and group B (implanted CPC/FG/BMSCs). The specimens were taken at 4 and 8 weeks after operation, and the repair of bone defect was observed by radiology, histological staining and immunohistochemistry. Results: The progress of bone repair in group C was faster than that in group An and B at 4 and 8 weeks after operation, and the immunohistochemical analysis showed that the positive expression of ALP in group C was higher than that in group B and group An at 4 weeks after operation. At 8 weeks after operation, the positive expression of ALP in group C and group B was significantly higher than that in group A (P < 0.05). At the same time, there was no significant difference between group C and group B (P > 0.05). Conclusion: The preliminary evaluation of the in vivo repair experiment of radius defect in osteoporotic rats shows that the calcium phosphate cement / fibrin glue composite scaffold of load growth factor and bone marrow mesenchymal stem cells may have a good ability to repair osteoporotic bone defect, and its mechanism and efficacy should be further studied.