Objective：To study the difference of intestinal microorganisms between rabbit k nee osteoarthritis (KOA) model and normal rabbits. Methods： Fecal samples were collected from 12 KOA model rabbits and 8 normal rabbit controls in the laboratory of Guangxi University ofTraditional Chinese Medicine. Total DNA of intestinal flora was extracted and amplified. The second-generation 16SrRNA gene targeted sequencing technology was used to analyze the diversity and distribution of intestinal flora.Results：At the phylum level, the number of Firmicutes and Bacteroides was the highest,while at the genus level, the number of Ruminococcus and Oscillatoria was the highest, and the marker species of the KOA group was Proteus. Conclusion：There are some differences in intestinal bacteria between KOA model rabbits and normal test rabbits, and intestinal bacteria may be a potential biomarker of KOA. Keywoeds:16S rRNA；Gut microbiota； Flora structure；Knee osteoarthritis