1.Beijing University of Chinese Medicine Third Affiliated Hospital, General Hospital of PLA;2.General Hospital of PLA
[目的] 探讨分析染色剂亚甲蓝对体外不同细菌培养结果的影响。[方法] 体外制备8种标准菌株（金黄色葡萄球菌、表皮葡萄球菌、人葡萄球菌、大肠埃希菌、肺炎克雷伯菌、鲍曼不动杆菌、化脓链球菌和白色念珠菌）的菌悬液来模拟关节液细菌培养。所有标准菌株的菌悬液均稀释50倍并分为三组（每组5份），实验Ⅰ组添加0.1%亚甲蓝注射液，实验Ⅱ组添加0.05%亚甲蓝注射液，对照组添加0.45%无菌盐水。将三组混悬液分别接种于琼脂平板培养基上进行24小时定量培养，通过菌落计数比较三组培养基上的细菌生长情况。[结果] 0.1%亚甲蓝注射液和0.05%亚甲蓝注射液对人葡萄球菌和鲍曼不动杆菌均有抑菌作用，对另6种标准菌株均无抑菌作用。其中鲍曼不动杆菌的生长情况明显受到亚甲蓝注射液的抑制（P<0.001），并且抑制程度与浓度呈正比。人葡萄球菌的生长情况也同样受到抑制（P<0.001），但不同浓度注射液之间的抑制程度并无统计学差异。[结论] 亚甲蓝溶液对临床中某些关节假体周围感染的致病菌具有一定的抗菌作用。
[Objective] To explore and analyze the effect of disclosing agent methylene blue on bacterial culture results of different bacteria in vitro. [Methods] Eight isolates of reference strains of Staphylococcus aureus, Staphylococcus epidermidis, Staphylococcus hominis, Escherichia coli, Klebsiella pneumoniae, Acinetobacter baumannii, Streptococcus pyogenes, and Candida albicans were incubated on the plates. Each bacterial suspension was formed by 50-fold dilution before the test methylene blue (MB) solution was added. For each strain, bacterial suspension was divided into 3 groups (5 samples each) exposed either MB solution 0.1%, MB solution 0.05% or sterile non-bacteriostatic 0.45% saline. The antimicrobial property of MB solution was determined by measuring the bacterial density on agar plates incubated for 24h and comparing it with controls exposed to sterile non-bacteriostatic 0.45% saline. [Results] Of the MB 0.01% or MB 0.05% exposure, reference strains but no Staphylococcus hominis and Acinetobacter baumannii strains resulted in fewer colony-forming units compared with the sterile saline control. The bacterial density of Acinetobacter baumannii on agar plates were significantly inhibited by MB solution (P<0.001), which were correlative with concentration. The colony-forming units of Staphylococcus hominis was also fewer (P<0.001), but there was no statistical difference between two concentrations of MB solution. [Conclusion] MB has a certain antibacterial effect on some pathogenic bacteria in periprosthetic joint infection.