Abstract: [Objective] To observe in vivo kinematic results of long-term follow-up of single-level Bryan artificial cervical disc replacement (ACDR). [Methods] A retrospective analysis was performed on 38 patients who underwent single-level Bryan ACDR in our hospital from January 2010 to March 2013, and the range of motion (ROM) of operated level > 5° at the last follow-up. The long-term clinical efficacy was evaluated by comparing the JOA, NDI and VAS scores before operation and at the last follow-up. Cervical X-ray films were collected before operation and at the last follow-up, and the overall cervical ROM and curvature, ROM and curvature of operated level, ROM of adjacent level, flexion-extension center of rotation (COR) at operated level were measured and compared at two time points. The correlation between follow-up data and flexion-extension COR at operated level was analyzed at the last follow-up. [Results] At the last follow-up, JOA, NDI and VAS scores were significantly improved compared with those before operation (P < 0.05), overall cervical curvature and curvature of operated level were increased compared with those before operation (P < 0.05), overall cervical ROM, ROM of operated level, ROM of adjacent level and flexion-extension COR at operated level had no significant change compared with those before operation (P > 0.05), COR(Y) was negatively correlated with ROM and translation distance of the same level (P < 0.05), follow-up data related to COR(X) were not found (P > .05). [Conclusion] The clinical efficacy and imaging results of single-level Bryan ACDR were satisfactory after more than 7 years of follow-up, flexion-extension COR at operated level was maintained. The relationship between translation, ROM and COR(Y) at operated level was similar to that at normal cervical level at the last follow-up, which was helpful to simulate the physiological movement pattern of cervical spine.