Abstract: [Objective] To discuss the influence of the anatomical morphological parameters related to the sagittal configuration of the lateral femoral condyle on injury of anterior cruciate ligament (ACL). [Methods] A retrospective analysis was performed on 100 patients undergoing arthroscopic knee surgery in our hospital from January 2017 to January 2020. There were 50 patients with ACL injury as the injury group, and 50 patients with simple meniscus injury as the control group. The femoral lateral condyle index was measured and calculated by MRI sagittal images of the lateral condyle of the femur in both groups. Thirty patients in the two groups had complete X-ray data. The posterior condyle ratio of femur lateral condyle was measured and calculated on standard lateral radiographs, and the correlation between the two groups and the risk of ACL injury was evaluated. [Results] The femoral lateral condyle index was (0.68±0.04) in the injured group and (0.72±0.05) in the control group, and the difference was statistically significant (P＜0.05), the cut-off value was 0.725; The posterior condyle ratio of femur lateral condyle was (0.68±0.09) in the injury group and (0.76±0.06) in the control group, and the difference was statistically significant (P＜0.05), and the cut-off value was 0.652. [Conclusion] The lateral condyle index and posterior condyle ratio of lateral condyle are predictive risk factors for an ACL injury. The risk of ACL injury increased when Sagittal configuration of the lateral condyle index and posterior condyle ratio of lateral condyle were reduced.