Ribosome biogenesis (RiBi) plays an important role in the growth and proliferation of cell. Currently, RiBi is considered has a close relationship with malignant tumor and interfering the process of RiBi will inhibit tumor cells. Some factors involved in RiBi have been convinced that could be potential therapy targets. Besides, RNA PolⅠinhibitors have been developed and tested clinically. The research of RiBi may provide new therapeutic approaches for malignant therapy.