Abstract [Objective]: The intraoperative navigation technique was used to verify the feasibility and accuracy of the stress conduction analysis method in determining the pedicle screw placement path. Methods: The authors studied 132 patients who underwent lumbar pedicle screw fixation from June 2019 to August 2020. In the observation group, 66 cases were treated with the method of stress conduction analysis. The control group (66 cases) was treated with surgical navigation system. CT and MPR reconstruction were performed after operation. The accuracy of screw insertion was evaluated by Rampersaud A—D grading method. intraoperative blood loss, operation time, incision length ,sagittal position, transverse position deviation and the success rate of first puncture were analyzed. Results: Two groups of patients successfully completed surgery, a total of 528 pedicle screw placement, of which 264 in the observation group and 264 in the control group. There was no significant difference between the control group and the observation group in the precision of nail placement and the deviation accuracy of sagittal and transverse position (P> 0.05). There was no significant difference in blood loss, operation time and incision length between observation group and control group (P> 0.05) . The success rate of the first puncture in the observation group was significantly higher than that in the control group (p < 0.05).Conclusion: It is feasible to determine the path of pedicle screw placement by stress conduction analysis, and the accuracy of pedicle screw placement is high.