[Objective] This study intends to clarify whether the kinematic alignment technique can improve the trajectory of the bearing movement as well as the clinical outcome and radiological assessment during Oxford mobile bearing unicompartmental knee arthroplasty (Oxford MB UKA). [Methods] We retrospectively analyzed the patients who undertook UKA from January to June, 2019. There were 23 patients in the kinematic group with 30 knees and 25 patients in the conventional group with 30 knees. The clinical outcome, radiological assessment and intraoperative bearing movement mode and distance of the two groups were compared. [Results]There was no difference in baseline characteristics between the two groups (P>0.05). The bearing movement trajectory was more optimal in kinematic group(P<0.05), but the movelength was without difference(P>0.05). In clinical outcomes, the kinematic alignment technique could reduce the hemorrhage and operation time (P<0.05), but the difference of AKSS scores between the two groups was not significant(P>0.05). Radiological assessment results: The contiguity between the femoral and tibial prosthesis was lower in kinematic group(P<0.05), but there were no differences in postoperative femoral and tibial prosthesis varus and valgus angle, femoral prosthesis flexion and extension angle, tibial slope and the convergence angle of the prosthesis (P>0.05). [Conclusion] This study demonstrated that the kinematic alignment technique in Oxford MB UKA has similar prosthesis installation accuracy and clinical outcome, but lower hemorrhage volume and shorter operation time compared with conventional MP instrumentation. However, The contiguity between the femoral and tibial prosthesis is smaller, and the bearing movement trajectory is more optimal.