Abstract Objective: To explore the regulatory effect and mechanism of miR-494 on apoptosis and invasion of osteosarcoma cells.Methods: The transient transfection method was used to up-regulate/down-regulate the miR-494 level of osteosarcoma MG-63 cells, the CCK-8 method was used to detect the cell proliferation ability, the Transwell method was used to detect the cell invasion ability, and the scratch test was used to detect the cell migration ability. , Use Western blot to detect the expression level of the target protein.Results: After transient transformation, the expression of miR-494 in MG-63 cells was higher than that in the WT group (P<0.05). CCK-8 kit was used to detect the proliferation of MG-63 cells. The overexpression group was at 24h, 48h, and The proliferation level at 72h was lower than that of the WT group (P<0.05). The average cell number, cell transmembrane number and cell migration rate in the overexpression group were lower than those in the WT group (P<0.05); the mRNA and protein expression of CDK6 in the overexpression group were lower than those in the WT group.Conclusion: The high expression of miR-494 can inhibit the proliferation of osteosarcoma 143B cells, and can cause the reduction of CDK6 mRNA and protein expression levels.