The anterior cruciate ligament (ACL) of the knee is an important part of the kinematics and biomechanics of the knee. It is a major constraint on the anterior displacement of the tibia. Valus and varus stress also play a role, and in addition, it limits the inward rotation of the tibial plateau. After cadaver, surgery and radiological studies, it has been confirmed that there are two functional bands of the ACL, namely, the anterior medial band and the posterior lateral band. The PL band is located distally from the femoral origin of the AM band and inserted into the posterior lateral side of the tibial insertion of the ACL. Currently, there is little debate about the existence of two functional bands of the ACL. However, there are still many controversies about the exact location of the normal ACL anatomy lies and how many bundles need to be reconstructed after ACL injury. Due to the different measurement methods and research objects, the data measured by researchers differ greatly. However, regardless of the positioning of the ACL during reconstruction, the use of single-bundle or double-bundls reconstruction depends on the surgeon''s understanding of the anatomical and functional relationship of the ACL. In this paper, we will review the nerve, vascular, surgical signs and anatomical features of the anterior cruciate ligament (ACL) to provide anatomical basis for surgeons to use markers to determine the insertion point of femur and tibia during ACL reconstruction.