Abstract: [Objective] To analyze the safety and effectiveness of 3D printing assisted high tibial osteotomy in patients with medial compartment osteoarthritis of the knee.[Methods] We retrospectively analyzed the patients with medial compartment osteoarthritis of the knee treated in our hospital and Xinxiang first people""s Hospital from May 2017 to May 2019. The patients in the 3D group were treated with 3D printing assisted osteotomy (62 cases), while the patients in the conventional group were treated with traditional osteotomy (58 cases).Observation indicators included perioperative conditions, follow-up indicators and imaging evaluation.[Results] The perioperative indexes of the 3D group were significantly lower than those of the conventional group (P<0.05).The pain and function scores in the 3D group were significantly better than those in the conventional group (P < 0.05); the incidence of adverse reactions in the 3D group was significantly lower than that in the conventional group (P < 0.05).The improvement of image index in the 3D group was significantly better than that in the conventional group (P < 0.05).[Conclusion] The application of individualized 3D printing assisted high tibial osteotomy in patients with medial compartment osteoarthritis of the knee has satisfactory effects in restoring knee function, relieving pain symptoms and promoting osteotomy accuracy, and can effectively avoid the effect of surgical trauma on rehabilitation.