Objective: To investigate the expression of CBX8 in a mouse degeneration model and its effect on disc degeneration Methods: NP cells were isolated from healthy mouse intervertebral disc. After cell passage cultivation,RT-PCR was used to detect mRNA expression of CBX8, type Ⅱ collagen, and proteoglycans. The Degenerative disease models of mice were prepared and tested at 8,12 and 16 weeks after acupuncture, intervertebral space height, senescence of nucleus pulposus cells and expression of inflammatory mediators were observed, To evaluate the degree of intervertebral disc degeneration and the relationship between CBX8 and intervertebral disc degeneration in mice. Results: The primary cultured nucleus pulposus cells were chondroids, and type II collagen was stained positive , mRNA expressions of type Ⅱ collagen and proteoglycans decreased（0.002±0.001、0.23±0.02） with an increase of CBX8 expression（1.68±0.26）after 5 generations. To establish the model of intervertebral disc degenerative disease; There was no significant change in vertebral height and lateral degeneration in the normal control group and the sham operation group. The vertebral body of the operation group had a certain degree of degenerative lateral bending, the height of the vertebral body also decreased significantly. In addition, the content of type II collagen in the surgery group was significantly lower than that in the normal group and the sham operation group, which indicated that the postoperative degeneration of the mouse disc was caused. In contrast, CBX8 levels were higher in the surgery group Conclusion: CBX8 expression was significantly higher in the mouse model of degeneration and in the nucleus pulposus cells after multiple passages than in the normal intervertebral discs and nucleus pulposus cells, CBX8 is associated with the degeneration of nucleus pulposus cells.