Heterotopic ossification (HO) was characterized by pathological bone formation in normal soft tissue, which occurred clinically in ligaments, muscles and tendons around joints, and the dysfunction caused by HO would seriously affect the quality of life. The pathogenesis of HO is still unclear. Vascular endothelial cells could differentiate into bone cells from mesenchymal stem cell intermediates formed by endothelial mesenchymal transition (Endo-MT).Local inflammatory signaling factors or other factors in the tissue microenvironment could mediate the differentiation of endothelium-derived mesenchymal stem cells into chondrocytes and osteoblasts so as to induce HO.This paper will summarize the research progress of Endo-MT in HO.