Abstract Methods:The model of 3-5mm fracture above the epiphyseal plate of the right distal femur of young rabbits was made and randomly divided into 4 groups, 8 in each group. The plates and screws of the same specification were used to fix the fracture. Rabbits in each group were killed at 2, 4, 6 and 8 weeks after operation. Take out the specimen and measure the length of femur. The thickness of epiphyseal plate and the morphological changes of mast cells were measured. The left epiphyseal plate of distal femur was used as the control group. RESULTS AND CONCLUSION:After 2、4、6 weeks of internal fixation with sliding plate, the experimental group took out the plate, and there was no significant difference in the observation indexes between the experimental group and the normal control group. However, after 8 weeks of internal fixation with sliding plate, the plate was taken out. There was statistically significant difference between the observation group and the control group. On the premise of no injury to the epiphyseal plate caused by the sliding plate and screw, the sliding hole produced sliding effect with the growth of epiphyseal plate at the initial stage of cross epiphyseal plate internal fixation (≤ 6weeks), but had no significant effect on the growth and development of epiphyseal plate（P＞0.05）. However, with the passage of time（≥8week）, mast cells in epiphyseal plate increase in value and divide, and can reach the end of the sliding hole screw, which limits the growth and development of epiphyseal plate, and has an impact on the development of epiphyseal plate. Compared with the control group, there was significant difference（P＜0.001）. Key words:Sliding plate; Trans epiphyseal plate; Periepiphyseal fracture; Internal fixation; Epiphyseal plate thickness; Mast cell.