Purpose To discuss the clinical result of 3D printed osteotomy template assisted correction of adult cubitus varus. Methods The data of 25 adult cubitus varus, who received osteotomy correction and plate internal fixation in the study hospital during January 2011 and December 2017, were retrospectively analyzed. This cohort included 14 patients who admitted to hospital before December 2014, they received a procedure using traditional way of osteotomy combined with plate internal fixation and were defined as traditional osteotomy group. The other 11 patients, who admitted to hospital after January 2015, received a procedure of 3D printed templated assisted osteotomy and internal fixation and were defined as 3D printed template assisted osteotomy group. Data of gender, age, degree of cubitus varus, blood loss, long of surgery, time for bony union, carrying angle and Mayo Elbow Performance Score (MEPS) 1 year postoperatively were collected for analysis. Results Preoperatively, there was no difference in regarding to gender, age, degree of cubitus and MEPS. Patients in both group obtained a follow up longer than 12 months, and they didn’t differed with each other in bony union time and MEPS 1 year postoperatively. The operation time of 3D printed template assisted osteotomy group was 63 minutes(ranging from 50 to75 minutes), with a blood loss of 92 ml(ranging from 70 to 130ml), while the operation time of traditional osteotomy group was 89 minutes(ranging from 65 to 110 minutes), with a blood loss of 134 ml(ranging from 110-200 ml). There was a significant decrease of blood loss and operation time in templated assisted group(P<0.05). One year postoperatively, the carrying angle was 12.6 degree (ranging from 9.7-15.5 degree) in templated assisted osteotomy group, which was significantly larger than that of traditional osteotomy group(5.9 degree, ranging from -2.7 to 10.8 degree). Conclusion Compared to traditional way of osteotomy, 3D printed templated assisted ostomy provided compatible clinical results, but was associated with decreased operation time, blood loss and more excellent carrying angle in the surgical treatment of cubitus varus.