Objective：To compare the effect of liquid preserved meniscus with fresh-frozen meniscus and cryopreservation, lay a foundation for promoting the clinical application of meniscus allograft transplantation. Methods：60 medial meniscus of adult healthy pigs were collected from nearby farms, and randomly divided into 3 groups for liquid (TSMU) preservation, fresh-frozen preservation(-80 ° c) and cryopreservation (-196 ° c). Morphology and biomechanical analysis were performed respectively on the preserved meniscus at week 2, 4 and 8. Results：HE staining, Safrin O and toluidine blue staining shown that TSMU liquid preserved meniscus and cryopreservation meniscus were significantly better than fresh-frozen meniscus (P<0.05).Young''s modulus analysis showed the same result. Conclusion： Liquid preserved meniscus and cryopreservation meniscus are better than fresh-frozen meniscus, which is embodied in morphology and biomechanics.