[Objective] To investigate the effects of naringin on osteoclast apoptosis and related mechanisms.[Methods] RAW264.7 cell line was used to induce osteoclasts at a concentration of 100 ng/ml RANKL for 5 days. Different concentrations of naringin were used to intervene osteoclasts for 3 days. TRAP staining was used to observe the number of naringin on osteoclasts. The effects of different concentrations of naringin on bone resorption of osteoclasts were observed by scanning electron microscopy. Flow cytometry was used to detect the effect of different concentrations of naringin on osteoclast apoptosis. The effect of naringin on the expression of apoptosis-related genes BCL-2, BAX, Caspase-3 and MMP-9 by real-time fluorescence quantitative PCR. [Results]Compared with the control group, the concentration of naringin in each group effectively reduce the number of RANKL-induced osteoclasts (P<0.05), and the concentration of 200μg/ml naringin is the strongest ( P<0.01). The bone resorption area of osteoclasts in each naringin group was decreased (P<0.05), and the concentration of naringin at 200μg/ml had the strongest inhibition on bone resorption of osteoclasts (P<0.01). Cytometry showed that the concentration of naringin could increase the apoptosis rate of osteoclasts (P<0.05), and the concentration of 20μg/ml and 200μg/ml naringin was stronger than that of 2μg/ml naringin group. Compared with the naringin group at 0μg/ml, the relative expression levels of BCL-2 and MMP-9 in the naringin group were decreased (P<0.05); 200μg/ml naringin group was the most significant (P<0.01); The relative expression levels of BAX and Caspase-3 in dermatan group were increased (P<0.05), and the relative expression of Caspase-3 at 200μg/ml naringin group to be the optimal dosage with satisfying therapeutic effects. (P<0.01). [Conclusion] Naringin down-regulate BCL-2 and up-regulate BAX and caspase-3 mRNA expression, and promote osteoclast apoptosis, thereby reducing the number and activity of osteoclasts. Among them, the concentration of 200 μg/ml was the strongest.