Abstract：Objective To study the feasibility, safety and efficacy of Intertransverse thoracic interbody fusion (ITIF) via transverse approach based on a randomized controlled animal experiment and clinical case. Methods Twenty-four adult goats (body weight 35.67 kg (±2.39 kg) were randomly divided into ITIF (Intertransverse thoracic interbody fusion) group, ITIF plus pedicle fixation group, TTIF (Transforaminal thoracic interbody fusion) group, TTIF plus pedicle fixation group, with 6 goats in each group. The same experienced spine surgeon performed the above operation on the experimental animals. X-ray and CT were taken at the 12th week after the operation. The time required for the operation, the amount of bleeding during the operation and the fusion of bone grafts at the 12th week after the operation were compared between the four groups. All data were analyzed statistically；Combined with clinical cases, verify its practicality. Results The operation time and bleeding volume of ITIF group were less than TTIF, the difference was statistically significant (P < 0.05). At 12 weeks, there was fusion between the four groups, but the bone fusion was incomplete and the trabecular arrangement was not perfect. Although the fusion rate is high or low, there is no significant correlation between the surgical method and the fusion rate. Conclusion Compared with transforaminal thoracic interbody fusion (TTIF), Intertransverse thoracic interbody fusion (ITIF) can significantly reduce the operation time and intraoperative bleeding. The fusion rate is also acceptable. ITIF plus pedicle fixation group has good feasibility, safety and effectiveness, and can be used in clinical practice；Good clinical results can be achieved by using the intertransverse approach and thoracic interbody fusion for clinically appropriate cases.