[Objective]: The aim of this study is to compare clinical effect of tibial fixations of anterior cruciate ligament (ACL) using a sheathed interference screw alone and sheathed interference screw combined with a staple. [Methods]: We retrospectively studied 71 patients who underwent ACL reconstruction from January 2014 to August 2016 in the Affiliated Hospital of Shandong University of Traditional Chinese Medicine. Patients who met the exclusion and inclusion criteria were divided into two groups according to tibial fixations, 37 cases with sheathed interference screws, and 34 cases with sheathed interference screws combined with staples. Physical examinations, including Lachman test and axial shift test were performed pre-operatively and 1 and 12 months after surgery to evaluate knee stability. Subjective evaluations including the International Knee Documentation Committee 2000 (IKDC) knee function score, Lysholm score were used to assess knee function. [Results]: At the final follow-up, IKDC2000 scores of the two groups were 90.30?4.28 and 91.10?4.13 respectively, and the difference was not statistically significant (p=0.932). The Lysholm scores of the two groups were 89.65?5.76 and 88.06?5.99 respectively. The difference between the two groups was not statistically significant either (p=0.341). There was no significant difference between the Lachman test and the axial shift test (p=0.440 and p=0.887 respectively). There were 3 cases reporting kneeling pain in the group with sheathed interference screw combined with staples. No kneeling pain was recorded for the group fixed with sheathed interference screw. [Conclusion]: After 1 year of follow-up, sheathed interference screw fixation and fixation of interference screw combined with a staple are equivalent in terms of postoperative knee function and knee joint stability. However, combination of interference screw and staple may be associated with postoperatively kneeling pain.