Objective: To observe the clinical and imaging effects of anatomical locking plate combined with bone graft support in the treatment of comminuted cuboid fractures through long-term and medium-term follow-up data. Methods: From July 2013 to March 2017, a total of 15 patients with comminuted cuboid fractures treated by mini locking plate combined with bone graft support were retrospectively analyzed, including 9 males and 6 females, with an average age of 48 + 12 (21 - 65 years). Preoperative CT and three-dimensional reconstruction scans were performed to confirm the comminuted fracture of the cuboid. At the last follow-up, the length (L), height (H), width (W), lateral longitudinal arch angle (LA), calcaneal fifth metatarsal angle (CM) of the cuboid on the standing X-ray film were measured and compared with those of the healthy side foot. The Maryland Foot Score and AOFAS Foot Score of the American Association of Foot and Ankle Surgery were used to evaluate the clinical function. Result: The average follow-up time was 3.4 +1.6 (2.1-5.8 years). Anatomical reconstruction was achieved in all the affected feet. No arch collapse and forefoot abduction were found during the follow-up. At the last follow-up, the length (L) of the cuboid bone was 31.41 (+1.14 mm), the height (H) was 30.69 (+2.20 mm), the width (W) was 24.59 (+2.57 mm), the average lateral longitudinal arch angle (LA) was 137.2 (+4.81) [130-143] [and the fifth metatarsal angle (CM) of the calcaneus was 12.49 (+0.48) [11.6-13.3] [compared with the healthy feet, and there was no significant difference (P > 0.05). At the last follow-up, Maryland's foot score was 92.7 + 3.8 [87-99] and the foot score of AOFAS of American Foot and Ankle Surgery Association was 91.8 + 4.6 [83-99], which was significantly different from that before operation (P < 0.05). Conclusion: Mini locking plate combined with bone graft support can achieve anatomical reduction of comminuted cuboid fracture, and stable foot and good function can be obtained in long-term follow-up.