[Objective] To simulate the high-load environment when fighter pilots are taking ultra-high acceleration flight and special tactical actions, and to explore the influence of this environment on the structural function and potential mechanism of intervertebral disc degeneration in pilots. [Methods] Tis study included 30 Sprague-Dawley (SD) rats in all 3 groups. The 10 SD rats in +6Gz and +10Gz groups were modeling in animal centrifuge. Rat body weight-statistics, MRI imaging and HE-staining of L5-6 intervertebral disc were used to measurement and analysis. [Results] With the increase of load, the weight growth rate of rats was weakened, the water content and height of the intervertebral disc of rats was reduced and the annulus fibrosus structure was destroyed in HE-staining. [Conclusion] Intervertebral disc degeneration in rats is related to the influence of high load, and the degree of degeneration increases with the increase of load.