Objective To investigate the effect of TGF-β1 on the expression of LOXs after injury of the posterior cruciate ligament. Methods Thirty-six adult New Zealand white rabbits were selected and divided into control group, PCL injury group and PCL injury + TGF-β1 group according to the experimental method. The rabbits were sacrificed at 0d, 7d, 14d and 21d after operation. The cross-ligament tissue was used to detect the gene expression of LOXs family in three groups of rabbits at different times by RT-PCR. Results There was no significant difference in the expression of LOXs family between the three groups (P>0.05). However, the expression of LOXs in the PCL injury group was lower than that of the control group at 7 days, 14 days and 21 days after surgery (P<0.05).The expression of LOXs in PCL injury+TGF-β1 group was higher than that in the PCL injury group at the corresponding time point (P<0.05). Conclusion TGF-β1 plays an important role in improving the healing ability ofPCL by regulating the expression of LOXs .