Abstract 【Objective】Contrast-enhanced ultrasound (CEUS) was used to quantitatively analyze the improvement of blood perfusion in soft tissue wounds treated by vacuum sealing drainage technique, and to explore the possible mechanism of the method. 【Methods】 In the rabbit model of deep soft tissue defect, intermittent VSD negative pressure suction was used as the experimental group, and gauze dressing was applied as the control group. Two days after treatment, the absolute peak intensity of blood flow, the relative velocity of blood flow enhancement and the changes of blood perfusion were quantitatively analyzed by contrast-enhanced ultrasound.【Results】 Two days after treatment, the Absolute Peak Intensity (API) of VSD group increased (P < 0.01), while that of gauze group decreased (P < 0.01). The API of VSD group was significantly higher than that of aseptic gauze group (VSD 13.43 (+1.53 dB), gauze 3.55 (+0.7 dB, P < 0.01); The Time to Peak(TTP) of VSD group did not change significantly before and after treatment (P = 0.63), gauze group prolonged (P < 0.01), and the TTP of VSD group was shorter than gauze group. The blood perfusion in VSD group was increased (P < 0.01) and gauze group decreased (P < 0.01). The blood perfusion in VSD group was significantly higher than that in aseptic gauze group (VSD 247.56 < 53.75 dB×s, gauze 64.18 < 7.91 dB×s, P < 0.01).【Conclusion】 Quantitative analysis of contrast-enhanced ultrasound (CEUS) proved that negative pressure sealing drainage can effectively improve the blood perfusion of deep soft tissue of wound, which may be one of the important mechanisms of this technique in wound repair.