[Objective] To investigate the quantitative evaluation of neurophysiological monitoring in different degrees of spinal cord injury (SCI).[Methods] 50 female healthy SD rats were randomly divided into 5 groups. Rats of control group only exposed T10 spinal cord. In the experimental group(40g?cm group, 60g?cm group, 80g?cm group, 100g?cm group), the self-made modified Allens strikers were used to strike the T10 spinal cord from 2cm, 3cm, 4cm, and 5cm of the rats respectively, resulting in different degrees of spinal cord injury. Using the Medtronic NIM Eclipse32 intraoperative nerve monitoring system to monitor the Transcranial electrical stimulation of motor evoked potential (TcMEP) and somatosensory evoked potential (SSEP), the waveforms was recorded preoperative, postoperative, 3 days, 1 week, and 2 weeks after the operation. And latency and peak amplitude were measured. At the same time, the observation score of the motor function of rats was performed.[Results] In this experiment, there was no spinal cord injury in the control group, and SSEP and TcMEP results were normal. When the spinal cord was hit by a lighter degree, the latency was prolonged and the amplitude decreased. When the spinal cord was severely hit, the latency was apparently prolonged，the amplitude significantly drop even disappears and the difference was significant (p<0.01). The motor function score showed that the score decreased significantly with the severity of the attack, and the difference between the groups was significant (p<0.01). After the operation, all the groups except 100g?cm had different degrees of waveform and motor function recovery, with significant differences between and within the groups (p<0.01). The TcMEP amplitude change of the spinal cord injury model was well correlated with the motor function score (p<0.01).[Conclusion] The neurophysiological monitoring can judge the severity of SCI accurately and objectively, and provide an effective experimental basis for the quantitative evaluation and prognosis of SCI.